The symposium is addressed to all scientists with experience and basic knowledge or who would like to gain such experience in extraction methods and chromatography as well as in high pressure and subcritical water extraction. - The mutual exchange between users and manufacturers of analytical equipment will encourage the solution of various problems. Different sample preparation methods e.g. with supercritical fluids, subcritical water, pressurized solvents as well as SPME, microwave extraction, and traditional extraction methods will be presented.